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Tuesday, May 19, 2020 | History

2 edition of interaction of fluorescent probes with mitochondrial membranes. found in the catalog.

interaction of fluorescent probes with mitochondrial membranes.

Nigel Anthony Edward Gains

interaction of fluorescent probes with mitochondrial membranes.

by Nigel Anthony Edward Gains

  • 204 Want to read
  • 16 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) - University of East Anglia, School of Biological Sciences, 1974.

ID Numbers
Open LibraryOL13845502M

The use of the fluorescent probe α-parinaric acid to determine the physical state of the intracytoplasmic membranes of the photosynthetic bacterium, Rhodopseudomonas sphaeroides. Biochimica et . In mitochondria, the chaperone Hsp70 functions as a molecular motor, pulling the precursor across the mitochondrial membranes; % of plant mitochondrial presequences contain an Hsp70 binding .

  MTT may, however, interact with the outer side of the inner mitochondrial membrane. A decrease in the fluorescence of TMRE and a monomeric form of JC‐1, which accompanied the reduction of MTT, may reflect dislodgment of the molecules of these probes from the mitochondrial membrane Cited by: CellLight Mitochondria-GFP (C, C, Figure ) and CellLight Mitochondria-RFP (C, C) reagents combine the utility and selectivity of targeted fluorescent proteins with the efficiency of the BacMam gene delivery and expression BacMam expression vectors encode Green Fluorescent Protein (GFP) or Red Fluorescent .

Combine targeting sequence–fluorescent protein fusion with the transduction efficiency of BacMam to label organelles independently of function (i.e., pH, mitochondrial membrane potential). Recognize . The fluctuation of the levels of mitochondrial thiols has been linked to many diseases and cells’ dysfunction. Therefore, the monitoring of mitochondrial thiol status is of great significance for clinical studies. We report here a novel fluorescence resonance energy transfer based two-photon probe MT-1 for mitochondrial Author: Zhengkun Liu, Qianqian Wang, Hao Wang, Wenting Su, Shouliang Dong.


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Interaction of fluorescent probes with mitochondrial membranes by Nigel Anthony Edward Gains Download PDF EPUB FB2

The Interaction of Phenothiazine Derivatives with Synaptosomal Membranes: the Use of Fluorescent Probes as an Indicator of Drug-Induced Conformational Perturbations. International Journal of Neuroscience Cited by:   Interaction of Fluorescent Probes with Hemoglobin Free Erythrocyte Membranes The Design of Fluorescent Probes for Membranes Conformational Activity vs.

Ion-Induced Osmotic Perturbation Book Edition: 1. These probes provide opportunities to investigate mitochondrial morphology and functions. However, such electrostatic interactions are not always reliable since the mitochondrial potential is highly changeable with mitochondria status.

The probes will leave when the mitochondria Cited by: 6. Probes of Structure and Function of Macromolecules and Membranes, Volume I: Probes and Membrane Function discusses the developments in techniques for the measurement of rapid.

By allowing for a decrease in membrane potential, caused by incorporation of cationic dye into the lipid bilayer, a modification of the Tomov theory predicts the dye titration data. We present a model of cationic dye-mitochondria interaction and discuss the use of these as probes of mitochondrial membrane Cited by: The development of techniques allowing high‐resolution analysis of mitochondria without destroying the living cell, combined with the emergence of fluorescent probes (timeline, Fig.

1), allows the study of mitochondria and mitochondrial Cited by:   Studying interactions between lysosomes and mitochondria in living cells is difficult due to the limitations of existing probes. Here, the authors develop new cell-permeable fluorescent probes Cited by: Thus, fluorescent chromophores can be selectively placed in different transverse regions of a model membrane system.

Full text Get a printable copy (PDF file) of the complete article (M), or click on Cited by: On the other hand, mitochondrial membrane-potential-responsive mitochondria targeting fluorescent probes, which usually bind strongly with mitochondrial proteins and form stable Cited by: 4. NBD-labelled lipids have been widely used as fluorescent probes of biological and model membranes, although somewhat criticized [16].

However, we obtained similar results with other fluorescent probes (dansyl, pyrene) or with quenching agents (brominated phospholipids).Cited by: 1. Furthermore, JC-1, a commercially available fluorescent probe, was used to measure the mitochondrial membrane potential (MMP) of HCT cells.

In healthy cells, JC-1 accumulates in the mitochondria Cited by: 6. Visualizing and tracking mitochondrial dynamic changes is crucially important in the fields of physiology, pathology and pharmacology. Traditional electrostatic-attraction based mitochondrial probes fail to visualize and track the changes due to their leakage from mitochondria when mitochondrial membrane.

A dicationic probe (Cz-Bz-1) was presented with excellent photostability and large Stokes shifts, which could migrate from mitochondria to the nucleolus and report mitochondrial membrane potential Author: Meng-Yang Li, Yan-Hong Liu, Kun Li, Hong Zhang, Lei Shi, Xin-Yao Liu, Kang-Kang Yu, Xiao-Qi Yu.

Fig. Photoswitching behavior of small-molecule probes for four membrane structures in live cells. BS-C-1 cells were labeled with (A) DiI for the plasma membrane, (B) MitoTracker Red for mitochondria, (C) ER-Tracker Red for the ER, (D) LysoTracker Red for row: Images taken with weak nm illumination to excite fluorescence from these probes Cited by: Permeant cationic fluorescent probes are shown to be selectively accumulated by the mitochondria of living cells.

Mitochondria-specific interaction of such molecules is apparently dependent on the Author: Nikolai Vekshin. 8-Anilinonaphthalenesulphonate interaction with whole and disrupted mitochondria: a re-evaluation of the use of double-reciprocal plots in the derivation of binding parameters for fluorescent probes binding to mitochondrial by: In recent years a large number of reviews, books, and proceedings of symposia on the application of fluorescence techniques to the study of a variety of biological problems have appeared.

This may be Cited by: Review Fluorescent Probes for Nanoscopic Imaging of Mitochondria Soham Samanta, 1,3Ying He, Amit Sharma,2 Jiseon Kim,2 Wenhui Pan, Zhigang Yang, 1,* Jia Li, Wei Yan, 1Liwei Liu, Junle Qu, 1,* and Jong Seung Kim 2 * Studying the ultra-fine structure and functions of mitochondria.

Studying mitochondrial structure and functions at the nanoscale level can shed light on many unresolved biological questions that have direct implications on many complicated health issues. This review highlights the critical aspects of choosing various nanoscopic methods for studying mitochondrial Cited by: 4.

8 Anilinonaphthalene 1 sulphonate interaction with whole and disrupted mitochondria: a re evaluation of the use of double reciprocal plots in the derivation of binding parameters for fluorescent.

Thus, the phospholipid-like Mito-MOI locates at mitochondrial inner membrane through electrostatic interaction between its cation and inner membrane negative charge.

Simultaneously, the C 18 -alkyl chain, as the second targeting group, is deeply embedded into the hydrophobic region of inner membrane through hydrophobic by: Molecular Dynamics Modeling of the Interaction of Cationic Fluorescent Lipid Peroxidation-Sensitive Probes with the Mitochondrial Membrane.

Nesterenko AM(1)(2), Kholina EG(3), Lyamzaev KG(1), Cited by: 2.The use of fluorescent probes to give continuous readouts of the structural states of mitochondrial membranes during energy coupling seems a logical extension of their use in the study of protein structural changes. causing a decreased volume of the structured water region and increased interaction Cited by: